Table 2.
Assignment of NO2-PhIP modified amino acid residues from SA that were identified by data-dependent MS/MS analysis
| Enzyme(s) | Fraction | Position | Peptide sequence | Modified site |
|---|---|---|---|---|
| Pronase E/leucine aminopediase/prolidase | 1 | N/A | N/A | Lysine |
| 2 | N/A | N/A | Cysteine | |
| 3 | N/A | N/A | Tyrosine | |
|
| ||||
| Trypsin | 1 | 191–197 | ASSAK*QR | Lys195 |
| 2 | 539–545 | ATK*EQLK | Lys541 | |
| 3 | 198–205 | LK*CASLQK | Lys199 | |
| 4 | 349–359 | LAK*TYETTLEK | Lys351 | |
| 5 | 411–413 | Y*TK | Tyr411 | |
| 6 | 30–41 | YLQQC*PFEDHVK | Cys34 | |
| 7 | 28–41 | AQYLQQC*PFEDHVK | Cys34 | |
| 8 | 138–144 | Y*LYEIAR | Tyr138 | |
| 9 | 390–402 | QNCELFEQLGEY*K | Tyr401 | |
| 10 | 149–159 | FY*APELLFFAK | Tyr150 | |
|
| ||||
| Trypsin/chymotrypsin | 1 | 191–197 | ASSAK*QR | Lys195 |
| 2 | 539–545 | ATK*EQLK | Lys541 | |
| 3 | 411–413 | Y*TK | Tyr411 | |
| 4 | 29–41 | LQQC*PFEDHVK | Cys34 | |
| 5 | 31–36 | LQQC*PF | Cys34 | |
| 6 | 149–155 | FY*APELL | Tyr150 | |
| 7 | 149–156 | FY*APELLF | Tyr150 | |
| 8 | 149–157 | FY*APELLFF | Tyr150 | |