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Assignment of HONH-PhIP modified amino acid residues from SA that were identified by data-dependent MS/MS analyses
| Enzyme(s) | Fraction | Position | Peptide sequence | Modified site |
|---|---|---|---|---|
| Pronase E/leucine aminopediase/prolidase | 1 | 34–36 | C*PF (sulfinamide) | Cys34 |
| 2 | 34–36 | C*PF (sulfonamide) | Cys34 | |
|
| ||||
| Trypsin/chymotrypsin | 1 | 31–41 | LQQC*PFEDHVK (sulfinamide) | Cys34 |
| 2 | 31–41 | LQQC*PFEDHVK (sulfonamide) | Cys34 | |
| 3 | 31–36 | LQQC*PF sulfinamide | Cys34 | |
