Table 2.

Summary of SP-PCR Data for Paired HNPCC Patient and Matched Normal Control PBL DNA at Three Loci

		D2S123				D5S346				D17S518				Three loci total and avg.
ID	PBL Age	n1	m2	F3	p-value4	n1	m2	F3	p-value4	n1	m2	F3	p-value4	n1	m2	F3	p-value4
N40	46	289	5	0.017	>0.05	276	2	0.007		257	1	0.004	>0.05	822	8	0.010	<0.01
H1	41	277	14	0.051		333	17	0.051	<0.01	214	3	0.014		824	34	0.041
N296	49	392	5	0.013	<0.05	581	0	0.000	<0.01	409	1	0.002	>0.05	1382	6	0.004	<0.01
H2	48	205	11	0.054		138	25	0.181		168	4	0.024		511	40	0.078
N380	68	270	0	0.000	<0.01	384	0	0.000	<0.01	582	0	0.000	<0.01	1236	0	0.000	<0.01
H3	71	53	24	0.453		79	11	0.139		87	18	0.207		219	53	0.242
N378	36	172	3	0.017	<0.05	141	10	0.071	<0.01	178	5	0.028	<0.01	491	18	0.037	<0.01
H4	36	121	12	0.099		51	24	0.471		130	23	0.177		302	59	0.195
N170	46	142	3	0.021	<0.05	65	1	0.015	<0.01	173	2	0.012	>0.05	380	6	0.016	<0.05
H5	46	151	10	0.066		99	8	0.081		191	3	0.016		441	21	0.048
N374	59	83	3	0.036	>0.05	90	2	0.022	<0.01	128	8	0.063	<0.01	301	13	0.043	<0.01
H6	59	155	1	0.006		94	17	0.181		126	37	0.294		375	55	0.147
N20	46	267	1	0.004	<0.05	381	5	0.013	<0.01	219	9	0.041	<0.05	867	15	0.017	<0.01
H75	42	241	18	0.075		142	8	0.056		164	17	0.104		547	43	0.079

¹Estimated number of alleles (n),

²number of observed mutants (m),

³mutant frequency (F) = m/n,

⁴p-value = z-score significance,

⁵patient H7 was previously published as “Patient B” [Coolbaugh-Murphy et al., 2004].

PBL, peripheral blood leukocyte;

HNPCC, hereditary nonpolyposis colorectal cancer.