. Author manuscript; available in PMC: 2013 Jan 14.

Published in final edited form as: Hum Mutat. 2010 Mar;31(3):317–324. doi: 10.1002/humu.21190

Table 3.

Summary of SP-PCR Data for Sporadic CRC Patient, Matched Normal Control, and HNPCC Positive Control PBL DNA at Three Loci

		D2S123				D5S346				D17S518				3 loci total and avg.
ID	PBL Age	n¹	m²	F³	p-value⁴	n¹	m²	F³	p-value⁴	n¹	m²	F³	p-value⁴	n¹	m²	F³	p-value⁴
N264	66	88	5	0.057		79	1	0.013		95	0	0.000		262	6	0.023
S1	65	478	5	0.010	>0.05	259	1	0.004	>0.05	332	5	0.015	<0.05	1069	11	0.010	>0.05
S2	70	190	4	0.021	>0.05	123	4	0.033	>0.05	252	0	0.000	>0.05	565	8	0.014	>0.05
H2 or H3⁵	48, 71	31	16	0.516	<0.01	38	6	0.158	<0.05	176	32	0.182	<0.01	245	54	0.220	<0.01
N380	68	132	5	0.038		259	6	0.023		177	2	0.011		568	13	0.023
S3	75	109	2	0.018	>0.05	183	8	0.044	>0.05	149	3	0.020	>0.05	441	13	0.029	>0.05
S4	80	198	8	0.016	>0.05	411	3	0.007	>0.05	248	5	0.020	>0.05	1157	16	0.014	>0.05
H2 or H3⁵	48, 71	62	8	0.129	<0.05	53	10	0.189	<0.01	104	9	0.087	<0.05	219	27	0.123	<0.01
N343	67	102		0.020		89	0	0.000		83	0	0.000		274		0.007
S5	72	517	4	0.008	>0.05	253	6	0.024	<0.01	358	2	0.006	>0.05	1128	12	0.011	>0.05
S6	68	59	5	0.085	>0.05	300	5	0.017	<0.05	75	3	0.040	<0.01	434	13	0.030	<0.05
H2 or H3⁵	48, 71	65	14	0.215	<0.05	35	6	0.171	<0.01	96	14	0.146	<0.01	196	34	0.173	<0.01
N343	67	22	1	0.045		48	3	0.063		28	0	0.000		98	4	0.041
S7	76	281	0	0.000	>0.05	66	2	0.030	>0.05	246	3	0.012	<0.05	593	5	0.008	>0.05
H2 or H3⁵	48, 71	21	4	0.190	<0.05	35	10	0.286	<0.05	46	7	0.152	<0.05	102	21	0.206	<0.01
N406	67	128	7	0.055		179	7	0.039		150	6	0.040		457	20	0.044
S8⁶	73	130	5	0.038	>0.05	123	0	0.000	>0.05	144	6	0.042	>0.05	397	11	0.028	>0.05

Estimated number of alleles (n),

number of observed mutants (m),

mutant frequency (F) = m/n,

⁴

p-value = z-score significance,

⁵

for positive controls: H3 for D2S123 and D17S518; H2 for D5S346,

⁶

patient S8 was previously published as “Patient C” [Coolbaugh-Murphy et al., 2004]. CRC, colorectal cancer.