Figure 1.
Kdm2b promotes iPSC generation. (a) RT–qPCR analysis of the expression levels of Kdm2a and different isoforms of Kdm2b (IF1, IF2 and IF3) in mouse ESCs and MEFs. Kdm2b-IF1 is highly expressed in mouse ESCs. The qPCR results were normalized to Gapdh and compared with the expression level in MEFs. (b) Kdm2b increases the efficiency of iPSC generation when co-introduced with OSK. The efficiency is represented by the number of Oct4–GFP+ colonies counted at post-transduction days 12 and 16 from 1 × 105 starting MEFs. n = 3. Error bars, s.e.m. (c) Kdm2b increases the reprogramming efficiency in the presence of c-Myc. Shown are numbers of Oct4–GFP+ colonies at day 12 reprogrammed by OSKM in the presence or absence of Kdm2b. n = 3. Error bars, s.e.m. (d) The reprogramming kinetics from day 6 to day 16 using different combinations of reprogramming factors. n = 3. Error bars, s.e.m. (e) RT–qPCR analysis of the knockdown efficiency of Kdm2b (Kdm2b-i). The results were normalized to the Gapdh level and are shown relative to the control shRNA treatment (Control-i). (f) Knockdown of Kdm2b reduced OSK-mediated reprogramming efficiency. Oct4–GFP+ colony numbers at days 14 and 18 are shown for OSK reprogramming with control or Kdm2b knockdown. Data in a, e and f represent the mean of two independent experiments.