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. 2001 Jul 3;98(14):7910–7915. doi: 10.1073/pnas.141223398

Figure 2.

Figure 2

The expression of the transgenic hCAR in B lymphocytes, T lymphocytes, and dendritic cells confers enhanced susceptibility to Ad transduction. (a) PMA-stimulated splenocytes were infected with the indicated MOI (0, 10, or 100) of AdGFP. After 48 h, the live cells were analyzed by flow cytometry for the expression of GFP and for the presence of the B lymphocyte marker B220. (Upper) wild type (WT). (Lower) hCAR transgene (CAR). Percentage of GFP-positive cells: WT; 10 MOI, 20%; 100 MOI, 38%. CAR; 10 MOI, 55%; 100 MOI, 75%. (b) IL-2-stimulated splenic T cells were infected with the indicated MOI (0, 10, or 100) of AdGFP. The live cells were analyzed by flow cytometry for the expression of GFP and for the presence of the T lymphocyte marker CD3. Percentage of GFP positive cells: WT; 10 MOI, 24%; 100 MOI, 28%. CAR; 10 MOI, 52%; 100 MOI, 85%. (c) Mature in vitro generated dendritic cells were infected with the indicated MOI (0 or10) of AdGFP. After 48 h, the live cells were analyzed by flow cytometry for the expression of GFP and for the presence of the CD11c as a marker for mature DCs. Percentage of GFP-positive cells: WT; 10 MOI, 16%. CAR; 10 MOI, 81%.