Table 1.

Anti-XPG expression using nine different antigen retrieval pretreatment conditions in a breast cancer specimen

Pretreatment	% Cell staining at each Intensity				% Pos	Stromal staining
	0	1	2	3
#1	100	0	0	0	0	1+
#2	100	0	0	0	0	0
#3	2	78	20	0	98	1+
#4	10	90	0	0	90	0
#5	92	5	0	3	8	2+
#6	20	80	0	0	80	0
#7	10	90	0	0	90	1+
#8	3	94	3	0	97	1+
#9	100	0	0	0	0	0

% pos, percentage of positive cells. #1: untreated; 2#: proteinase K (Dako) for 5 minutes at room temperature (RT); #3: HIER using High Tide buffer for 40 minutes at 95°C in a water bath; #4: HIER using Red Tide buffer (Mosaic Laboratories) for 40 minutes at 95°C in a water bath; #5: HIER using Rip Tide buffer for 40 minutes at 95°C in a water bath; #6: HIER using Green Tide buffer (Mosaic Laboratories) for 40 minutes at 95°C in a water bath; #7: HIER using Diva buffer (Biocare Medical, Concord, CA) for 40 minutes at 95°C in a water bath; #8: HIER using Diva buffer with a decloaker set for 30 seconds at 125°C; #9: offshore Buffer (Mosaic Laboratories) for 10 minutes at RT.