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. Author manuscript; available in PMC: 2013 Jan 14.
Published in final edited form as: J Immunol. 2010 Sep 8;185(7):4496–4505. doi: 10.4049/jimmunol.1001980

FIGURE 5.

FIGURE 5

Rabbit anti–GBV-C E2 Abs precipitate HIV particles. HIV-1 particles were immune precipitated (IP) by the postimmune rabbit IgG (R1, R2, R3, and R4) but not preimmune (pre) rabbit anti-E2 IgG (A; *p < 0.01 compared with preimmune IgG). CHO cell culture supernatants containing E2 protein inhibited IP in a dose-dependent manner, whereas CHO cell culture supernatants without E2 did not (A). The protein concentration represents the total protein in culture supernatants and not E2-specific protein. Defective HIV gag particles displaying HIV, VSV, or GBV-C envelope glycoproteins, or HIV-1 particles with no virus envelope glycoproteins were precipitated by postimmune rabbit anti-E2 IgG and not by preimmune rabbit IgG (B; *p < 0.01 compared with preimmune IgG; †p < 0.05). YFV and mumps virus (MV) were not neutralized following incubation with pre- or postimmune (post) rabbit E2 IgG (left panel; C), and they were not IP by postimmune rabbit anti-E2 IgG (right panel; C). NA, no Ab control; IPV, the amount of input virus used in IP experiments.