Fig. 2.

Mutational analysis to locate the downstream EUO-binding site of the omcAB promoter.

A. DNA sequence of the wild-type (WT) EUO-binding site and mutant templates containing nucleotide substitutions (highlighted in bold). The –35 promoter element is underlined – note that the 9 bp substitution in M3 did not alter the sequence of this –35 promoter element.

B. EMSA experiments with DNA probes containing these wild-type or mutant sequences. EMSA reactions were performed with 0, 80 or 160 nM rEUO. Bands corresponding to the bound and free probes are indicated on the right.