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DNA fragments from late promoters, but not early or mid promoters, effectively compete for EUO binding. Competitive EMSAs were performed with the 60 bp labelled omcAB and 160 nM EUO in the presence of 50 nM unlabelled competitor DNA fragments (100-fold excess compared to the labelled omcAB probe). The promoter present on each DNA fragment is indicated for each reaction. Bands corresponding to the bound and free probes are indicated on the right.
