Table 1.
Binding and transcriptional repression of chlamydial promoters by EUO.
| Promoter | Bindinga | Repression (%)b | |
|---|---|---|---|
| Early genes | dnaK (ct396) | No | 6 ± 5 |
| groESL (ct110) | No | 21 ± 13 | |
| rRNA | n.t. | 7 ± 8 | |
| Mid genes | cdsC (ct674) | No | 12 ± 6 |
| fliF (ct719) | No | 11 ± 12 | |
| ct665 | n.t. | 21 ± 11 | |
| ompA (ct681) | n.t. | 12 ± 10 | |
| Late genes | omcB (ct443) | Yes | 60 ± 18 |
| ltuB (ct080) | Yes | 69 ± 9 | |
| ssc2 (ct576) | Yes | 75 ± 2 | |
| cdsU (ct091) | Yes | 46 ± 19 | |
| mraY (ct757) | Yes | n.t. | |
| ct783 | Yes | n.t. | |
| ct867 | Yes | n.t. | |
| ftsK (ct739) | Yes | n.t. | |
| ct356 | Yes | n.t. |
a
Binding was determined by EMSA using EUO protein over a range of 80–320 nM for each 60 bp labelled DNA probe containing the respective promoter.
b
Per cent repression was calculated as the ratio of transcript levels in the presence/absence of EUO. Results are from at least three independent experiments and are reported as the mean and standard deviation for each promoter.
n.t., not tested.