Table 2.
Symbol | Description | P88 Control | P98 (Adult Ethanol) | P148 Control | P148 (Adult Ethanol) |
---|---|---|---|---|---|
Peptide receptors | |||||
Cckar | Cholecystokinin α receptor | 100 | 108 | 66 | 44* |
Galr1 | Galanin receptor 1 | 100 | 45 | 33* | 43 |
Galr2 | Galanin receptor 2 | 100 | 79 | 111 | 45* |
Npffr2 | Neuropeptide FF receptor 2 | 100 | 70 | 21*§ | 36 |
Npy2r | Neuropeptide Y receptor Y2 | 100 | 94 | 33* | 65 |
Npy5r | Neuropeptide Y receptor Y5 | 100 | 89 | 39* | 80 |
Prokr2 | Prokineticin receptor 2 | 100 | 87 | 24*§ | 47 |
Tacr3 | Tachykinin receptor 3 | 100 | 54 | 31* | 51 |
Cholinergic receptors | |||||
Chrm3 | Cholinergic receptor, muscarinic 3 | 100 | 88 | 33* | 57 |
Chrm4 | Cholinergic receptor, muscarinic 4 | 100 | 83 | 38* | 79 |
Chrm5 | Cholinergic receptor, muscarinic 5 | 100 | 81 | 31* | 58 |
GABA receptors | |||||
Gabra4 | GABA-A receptor, α4 | 100 | 71 | 25*§ | 60 |
Gabrr2 | GABA-A receptor, ρ2 | 100 | 111 | 65 | 87 |
Gad1 | Glutamic acid decarboxylase 1 | 100 | 70 | 21*§ | 51 |
Adult alcohol binge slightly alters neurotransmitter gene expression across development. Mice received either water or ethanol (5g/kg) once a day for 10 days during adulthood (P88-97). The expression of 84 neurotransmitter receptor genes was assessed in whole brain either 24 hours after treatment (P98; N=6 Control, 6 Ethanol) or in adulthood (P148; N=5 Control, 6 Ethanol) using an RT2 Profiler Neurotransmitter Receptor and Regulator Superarray™. Gene expression levels are given as the percent of P88 control. Genes that changed in at least one treatment group are presented. A 2-way ANOVA with Bonferroni post-tests to account for multiple comparisons was used to determine statistical significance of individual genes (§ p<0.05). Student t-tests were also performed in order to gain insight into other genes that might be changing, however t-test analysis may produce type II errors.(t-test versus P88 control:
p<0.05).