Figure 3. .

Priming of ARPE-19 cells by inducers of NF-κB. (A) ARPE-19 cells grown to confluence on plastic wells were treated with the NF-κB–activating agents LPS, IL-1α, or TNFα at a concentration of 4 ng/mL or 50 ng/mL for 24 or 48 hours, or were left untreated as a negative control (Ctrl). (B, C) Confluent ARPE-19 cells were treated with increasing concentrations of IL-1α for 48 hours (B) or 4 ng/mL IL-1α for increasing lengths of time (C). ARPE-19 cell lysates were immunoblotted for pro-IL-1β, after which blots were stripped and reprobed for GAPDH as a loading control. Densitometry was performed using NIH ImageJ, and GAPDH-normalized band intensities are displayed for each condition.