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Flow cytometric binding analysis of Wx-Ki-4(scFv) fusion proteins on L540cy cells. (A) Ki-4(scFv), (B) W1-Ki-4(scFv), (C) W2-Ki-4(scFv) and (D) W3-Ki-4(scFv). The black curve represents L540cy cells incubated with only the secondary antibody (background). The grey curve represents the different versions of the Wx-Ki-4(scFv) proteins. The signal shift to the right means increased fluorescence intensity in relation to the black curve as the antibody binds to the CD30 receptor.
