Table 1.
Cytometric analysis of Plasmodium falciparum GFP+gametocytes (line 164/GFP) before and after sorting
Cell population | % GFP+cells-Exp1 | % GFP+cells-Exp2 | % GFP+cells-Exp3 |
---|---|---|---|
Before sorting |
0.05% |
0.06% |
0.03% |
After sorting | 95.1% | 94.7% | 93.1% |
Gametocytes were collected in an equal volume of PBS supplemented with 1% BSA, HEPES and L-glutamine, subsequently subjected to imaging cytometer analysis and quantified events were reported. Data are percentage of GFP+ gametocytes (from line 164/GFP) acquired with flow cytometer FACSAria (before sorting) and with imaging cytometer Imagestream 100 (after sorting).