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. 2012 Sep 5;11:312. doi: 10.1186/1475-2875-11-312

Table 1.

Cytometric analysis of Plasmodium falciparum GFP+gametocytes (line 164/GFP) before and after sorting

Cell population % GFP+cells-Exp1 % GFP+cells-Exp2 % GFP+cells-Exp3
Before sorting
0.05%
0.06%
0.03%
After sorting 95.1% 94.7% 93.1%

Gametocytes were collected in an equal volume of PBS supplemented with 1% BSA, HEPES and L-glutamine, subsequently subjected to imaging cytometer analysis and quantified events were reported. Data are percentage of GFP+ gametocytes (from line 164/GFP) acquired with flow cytometer FACSAria (before sorting) and with imaging cytometer Imagestream 100 (after sorting).