Table 1.

Differential Immunity profiles of each LES phage in PAO1

Efficiency of plating values	φ2	φ3	φ4
PAO1 naive host	1.0	1.0	1.0
Single φ2 lysogen	< 1x10 -9	< 1x10 -9	0.017
Single φ3 lysogen	0.91	< 1x10 -9	0.37
Single φ4 lysogen	1.09	0.94	3.3x10 -9

Immunity profiles of each LES phage were determined by plaque assay. Phage dilution series were spotted onto non-Lysogenic PAO1 and PLPL lawns. Efficiency of plating = the ratio of plaques observed (at the appropriate phage dilution) on the most permissive host (non-lysogenic PAO1)/plaques observed on assay host (PLPL harbouring LESφ2, LESφ3 or LESφ4 prophages). If no plaques were observed when neat phage suspensions of 10¹⁰ p.f.u ml^-1 were used, an eop value < 1x10^-9 was recorded.