Table 1.
Description of site and isolates collected, including isolation success and genes sequenced from each site
| |
Site |
Region |
Origin |
Date Collected |
No. cells isolated (survived) |
ITS1 sequences (#) |
18S and 28S Sequences (#) |
Lineage 1 |
Lineage 2 |
Lineage 3 |
Physiology |
|
|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
a.) |
1 |
North Pacific |
Queen Charlotte Islands, Canada 51.75°N, 131°W |
5/17/2007 |
21 (18) |
10 |
|
3 |
6 |
1 |
|
|
| 2 |
North Pacific |
Vancouver Island (A), Canada 49.65°N, 127.44°W |
5/13/2007 |
48 (41) |
10 |
1 |
10 |
|
|
1 |
||
| 3 |
North Pacific |
Vancouver Island (B). Canada 48.87°N, 125.89°W |
5/13/2007 |
32 (30) |
10 |
1 |
10 |
|
|
1 |
||
| 4 |
North Pacific |
Puget Sound, USA 47.74°N, 122.42°W |
5/12/2007 |
56 (56) |
10 |
1 |
|
10 |
|
|
||
| 5 |
North Pacific |
La Jolla, USA 32.85°N, 117.25°W (CCMP1018) |
1968 |
NA |
1 |
1 |
1 |
|
|
1 |
||
| 6 |
North Pacific |
Seto Inland Sea, Japan 34.16°N, 133.33°E |
2/2007 |
NA |
5 |
1 |
|
|
5 |
1 |
||
| |
7 |
North Atlantic |
Martha’s Vineyard Coastal Observatory, USA 41.45°N, 70.56°W |
11/3/2008 |
17 (15) |
10 |
|
|
|
10 |
|
|
| 8 |
North Atlantic |
Narragansett Bay, URI 41.53°N, 71.38°W |
10/10/2008 |
24 (24) |
10 |
|
|
|
10 |
|
||
| |
1/8/2009 |
24 (21) |
10 |
1 |
|
|
10 |
|
||||
| |
|
|
2/9/2009 |
48 (45) |
10 |
|
|
|
10 |
|
||
| |
|
|
6/26/2009 |
4 (2) |
2 |
|
|
|
|
|
||
| 9 |
North Atlantic |
Oban, Scotland 56.57°N, 5.43°W (CCAP1085_20) |
2008 |
NA |
1 |
1 |
|
|
1 |
|
||
| |
10 |
Mediterranean Sea |
Gulf of Naples, Italy 40.95°N, 14.25°E (CCAP1085_21) |
2008 |
NA |
1 |
1 |
|
|
1 |
1 |
|
| 11 |
Mediterranean Sea |
Gulf of Naples, Italy 40.49°N, 14.15°E (CCMP1647) |
11/12/2008 |
NA |
1 |
1 |
|
|
1 |
|
||
| 12 |
Mediterranean Sea |
Gulf of Naples, Italy 40.75°N, 14.33°E (CCMP3264) |
1993 |
NA |
1 |
1 |
|
|
1 |
1 |
||
|
b.) |
13 |
North Atlantic |
Iceland 60.92472°N, 27.005833° |
5/22/2008 |
48 (29) |
10 |
3 |
|
|
|
|
|
| |
14 |
North Atlantic |
Tromso, Norway 69.66°N, 18.96°E (CCMP986, 987) |
8/1/1978 |
NA |
2 |
1 |
|
|
|
|
|
| 15 | Southern Ocean | McMurdo Sound77.83°S 163.00°E, Antarctica (CCMP1463, CCMP1462) | 2/1/1991 | NA | 2 | 2 |
Site numbers refer to map featured in Figure 1. Isolation number or isolation success number was not available for culture collection strains, nor for those isolated in Japan. Culture collection accession numbers are indicated in the “Origin" column. a) All T. rotula isolates, including number classified as 1, 2, or 3, number of isolates included in physiological experiments. b) Description of T. gravida isolates.