Table 1. Sequences of AFLP and MSAP adapters and primers used in this study.
Adaptors | |
EcoR I-adaptors-F | 5′CTCGTAGACTGCGTACC3′ |
EcoR I-adaptors-R | 5′AATTGGTACGCAGTC3′ |
Mse I-adaptors-F | 5′GACGATGAGTCCTGAG3′ |
Mse I-adaptors-R | 5′TACTCAGGACTCAT3′ |
Hpa II/Msp I-adaptors-F | 5′GATCATGAGTCCTGCT3′ |
Hpa II/Msp I-adaptors-R | 5′CGAGCAGGACTCATGA3′ |
Preselective primers | |
EcoR I-A | 5′GACTGCGTACCAATTCA3′ |
Mse I-C | 5′GACGATGAGTCCTGAGTAAC3′ |
Hpa II/Msp I-T | 5′ATCATGAGTCCTGCTCGGT3′ |
Selective primer combinations used in AFLP | |
EcoR I-AAC+ Mse I-CTC | EcoR I-ACC+ Mse I-CAA |
EcoR I-AAC+ Mse I-CTG | EcoR I-AGG+ Mse I-CAA |
EcoR I-ACT+ Mse I-CTG | EcoR I-ACG+ Mse I-CAA |
EcoR I-ACA+ Mse I-CAA | EcoR I-ACG+ Mse I-CAA |
Selective primer combinations used in MSAP | |
EcoR I-AAC+ Hpa II/Msp I-TTCT (E1HM7) | EcoR I-AAG+ Hpa II/Msp I-TTGC (E2HM2) |
EcoR I-AAC+ Hpa II/Msp I-TCGA (E1HM1) | EcoR I-AAG+ Hpa II/Msp I-TCGA (E2HM1) |
EcoR I-AAC+ Hpa II/Msp I-TCCA (E1HM3) |