. 2013 Jan 14;8(1):e54057. doi: 10.1371/journal.pone.0054057

Table 3. Number of bands amplified using five MSAP selective primer combinations in the 11 materials used in this study.

Primer combinations^*			E1HM7	E1HM1	E1HM3	E2HM2	E2HM1	Total		Rate of methylated site (%)
Number of amplification site			31	19	26	27	31	134
Mianyang11	Methylated site	Fully^**	2	1	1	2	4	10	54	7.46	40.30
		Hemi^***	11	4	12	9	8	44		32.84
MY+2RF₂	Methylated site	Fully	4	2	5	6	4	21	51	15.67	38.06
		Hemi	8	1	6	5	10	30		22.39
MY+2RF₃	Methylated site	Fully	2	3	7	5	5	22	56	16.42	41.79
		Hemi	7	2	6	6	13	34		25.37
2RF₃MY-4	Methylated site	Fully	3	4	5	8	5	25	61	18.66	45.52
		Hemi	10	6	3	4	13	36		26.87
2RF₃MY-8	Methylated site	Fully	1	1	3	8	8	21	65	15.67	48.51
		Hemi	13	4	12	7	8	44		32.84
2RF₃MY-10	Methylated site	Fully	7	3	7	5	7	27	63	20.15	47.01
		Hemi	8	6	8	7	7	36		26.87
MY+5RF₂	Methylated site	Fully	4	2	5	5	7	23	46	17.16	34.33
		Hemi	11	2	7	2	1	23		17.16
MY+5RF₃	Methylated site	Fully	8	4	5	9	8	34	63	25.37	47.01
		Hemi	10	1	4	4	10	29		21.64
5RF₃MY-1	Methylated site	Fully	8	1	3	10	8	30	52	22.39	38.81
		Hemi	6	2	4	5	5	22		16.42
5RF₃MY-5	Methylated site	Fully	4	1	3	4	2	14	52	10.45	38.81
		Hemi	6	0	11	6	15	38		28.36
5RF₃MY-11	Methylated site	Fully	4	0	4	3	8	19	65	14.18	48.51
		Hemi	6	12	9	8	11	46		34.33

The names of primer combinations are abbreviations and their corresponding full name are listed in Table 1.

^**

The band pattern that absent for Hpa II but present for Msp I was regarded as full-methylation and the numbers of this kind of band pattern amplified by each primer pairs were scored in each material.

^***

The band pattern that for present Hpa II but absent for Msp I was regarded as hemi-methylation and the numbers of this kind of band pattern amplified by each primer pairs were scored in each material.