| Crystallization conditions |
22–24% PEG 3350, 0.2 M Ammonium sulfate, 0.1 M bis-tris pH 6.6, 10–20mM TCEP (pH 7), 13°C |
12–15% PEG 400, 0.1 M Bicine pH 9, 25 mM TCEP (pH 7) |
| Data collection |
| Beamline |
APS 17ID |
APS 17ID |
| Resolution |
1.31 |
3.23 |
| Space group |
P21
|
P4 |
| Unit cell parameters (Å, o) |
71.1, 42.3, 92.6; β = 98.6 |
145.5, 145.5, 103.7 |
| Contents of the asymmetric unit |
Two kinase domains |
Four kinase+SH3 domains |
| Measured reflections |
472,369 |
227,669 |
| Unique reflections |
124,856 |
34,878 |
| Data redundancy |
3.7 |
6.5 |
| Data completeness (%) |
97.7 (93.6) |
99.5 (96.7) |
| Rmerge
|
0.107 (0.581) |
0.187 (0.477) |
| I/σ |
11.2 (2.3) |
9.9 (2.0) |
| Refinement |
| Rwork/Rfree (%) |
19.5/20.9 |
24.6/27 |
| Number of protein atoms |
4,270 |
10,200 |
| Number of non-protein molecules |
496 water, 2 SO4
−2, 2 ethylene glycol |
0 |
| RMSD, bond lengths (Å) |
0.003 |
0.009 |
| RMSD, bond angles (o) |
0.8 |
1.1 |
| PDBID |
4HZR |
4HZS |
