Table 2. Kinetic characterization of Ack1.
| Enzymea | kcat (min−1) | Km (ATP, µM) | kcat/Km(µM−1 min−1) |
| GST-CD | 515±140 | 240±90 | 2.1 |
| P-GST-CD | 332±13 | 120±70 | 2.8 |
| CD | 26±2 | 140±30 | 0.19 |
| P-CD | 65±2 | 210±40 | 0.31 |
| KD | 16.5±0.6 | 134±9 | 0.12 |
| P-KD | 32±3 | 177±9 | 0.18 |
| KD+SH3 | 10±0.3 | 140±10 | 0.07 |
| P-KD+SH3 | 22±2 | 181±4 | 0.12 |
a
GST-: GST-tagged protein; P-: phosphorylated protein; CD: residues 110–476 spanning kinase domain, SH3 domain and CRIB region; KD: residues 115–389 spanning the kinase domain; KD+SH3: residues 115–453 spanning kinase and SH3 domains.
Values are averages ± SD.