Figure 3. Deletion of the TDH2 gene restores both CLS and vegetative growth and reduces the metabolic intermediates of glucose metabolism in hst3Δ hst4Δ cells.

(A) The CLSs of wild type, hst3Δ hst4Δ, tdh2Δ and tdh2Δ hst3Δ hst4Δ cells. (B) The CLSs of wild type, hst3Δ hst4Δ, tdh2Δ and tdh2Δ hst3Δ hst4Δ cells. (C) The CLSs of wild type, hst3Δ hst4Δ, tdh2Δ and tdh2Δ hst3Δ hst4Δ cells. (D) Growth curves of wild type, hst3Δ hst4Δ, tdh2Δ and hst3Δ hst4Δ tdh2Δ cells in SC medium. (E) The metabolic profiling data of central carbon metabolism in wild type, hst3Δ hst4Δ, tdh2Δ and hst3Δ hst4Δ tdh2Δ cells were mapped onto known pathways. Each graph was derived from the calculated amount of metabolic intermediates shown in Table S3.