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. 2013 Jan 14;8(1):e53991. doi: 10.1371/journal.pone.0053991

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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, 28 hpf embryos. A, wild-type (wt), B, med12^y82 mutant, and C, wt embryo treated at sphere stage with 1.5% ethanol for 24 hrs. Note the similarly retarded brain development in the med12^y82 and ethanol treated embryos (arrows). D−I, ventral views at 72 hpf of Tg(gsc:GFP) embryos (D, E, F) and med12^y82; Tg(gsc:GFP) embryos (G, H, I). D, G, control embryos. E, H, treated with 1% ethanol for 36 hrs at 24 hpf. F, I, treated with 2% ethanol for 36 hrs at 24 hpf. Embryos and eyes are outlined (dashed white lines). J−O, Tg(isl1:GFP) embryos (J, K, L) and med12^y82; Tg(isl1:GFP) embryos (M, N, O) at 56 hpf, dorsal views. J, M, control embryos. K, N, treated with 1% ethanol for 24 hrs at 24 hpf. L, O, treated with 2% ethanol for 24 hrs at 24 hpf. GFP-expressing cranial motor neurons (labeled) diminished in med12^y82; Tg(isl1:GFP) embryos, especially in nuclei III and IV. A reduction of nucleus V neurons in med12^y82; Tg(gsc:GFP) embryos treated with ethanol is illustrated by the elimination of the prominent nV axons (arrows). In contrast, the prominent nVII axons remained intact (arrowheads). Otic capsule (oc), tectum (te).