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. 2013 Jan 14;8(1):e53991. doi: 10.1371/journal.pone.0053991

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Whole mount in situ hybridization was used to detect: A, GFP; B, oxtl; and C, tyrosine hydroxylase (th) mRNAs in Tg(oxtl:GFP) larva treated with ethanol for 24 hrs at 4 dpf, and then fixed at 6 dpf. th labels dopaminergic and noradrenergic neurons located in the area postrema. Examples of positive staining are indicated by arrows.