Figure 5.

Whole-cell hydroxylation of different steroids. Whole-cell hydroxylation of different steroids in aqueous phase system, employing Y. lipolytica YL21 and YL22 cells harboring CYP2D6 (filled bars) or CYP3A4 (striped bars), respectively. Data is shown as activity relative to the highest measured apparent hydroxylation rate of testosterone (3.9 ± 0.1 mU per g CDW). Compounds were converted by cells equivalent to ~ 10 g/L CDW for 16 hours. Activity was determined in test tubes as product formation rate after adding steroids dissolved in DMSO to a final concentration of 2 mM.