Figure 3.

IL-4 and pyridon 6 do not interfere with MAP kinase pathways. Lymphocytes were preincubated for 30 min with/without pyridon 6 and stimulated for 2 h with IL-4. Cell lysates were subjected to Western Blotting followed by densitometry. Representative immunoblots show p42 and p38 phosphorylation; loading was controlled by staining for non-phosphorylated p44/42 (42/44 kDa) and non-phosphorylated p38 (38 kDa), respectively. Densitometry results are given as mean % expression of loading control ± SEM in the bar graphs; statistical analysis was performed using the Friedman and Dunn’s test; *P < 0.05. The number of independently performed experiments was n = 4 for p42 and n = 3 for p38 phosphorylation.