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. 2012 Nov 13;8:83. doi: 10.1186/1744-8069-8-83

Figure 4.

Figure 4

Cellular levels of beta-endorphin immunoreactivity increase in ConA-activated and IL-4-stimulated lymphocytes.A) 24 h after cell stimulation with/without IL-4 (10 ng/ml) and ConA (1 μg/ml), beta-endorphin immunoreactivity was determined in cell lysates. Data represent the beta-endorphin immunoreactivity in pg/1.0E+06 cells of n = 6 independent experiments; median is indicated by the horizontal line. B) Ionomycin-induced release of immunoreactive beta-endorphin was determined using EIA in the supernatant of cells stimulated for 24 h with/without IL-4 (10 ng/ml), ConA (1 μg/ml), and pretreated with pyridon 6 at the indicated doses. Data represent the beta-endorphin immunoreactivities in pg/1.0E+06 cells of n = 8 independent experiments; median is indicated by the horizontal line. All data were analyzed using Friedmann and Dunn’s Test in comparison to unstimulated controls (*P < 0.05).