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. 2013 Jan 14;8(1):e53901. doi: 10.1371/journal.pone.0053901

Figure 3. SSITL silenced transformants showing abnormal phenotypes.

Figure 3

(A) The construction of SSITL silenced vector. (B) Northern blots analysis of the SSITL gene transcript accumulation in SSITL silenced transformants. Expression of SSITL in the wild-type Ep-1PNA367 served as control. Hyphae mass from 3-day-old colonies on PDA was collected for gene expression analysis. RNA samples were monitored by Northern hybridization analysis of 18S rRNA on the nylon membrane. (C) Abnormal colony morphology produced by SSLTL silenced transformants. Colonies were grown on PDA for 30 days at 20°C. (D) Excessive branching of hyphal tips of SSITL silenced transformants. (E) Hyphal growth rates of SSITL silenced transformants. Growth rates were examined on PDA at 20°C. Different letters in the graph indicate statistical differences, P = 0.01. (F) Virulence decreases in SSITL silenced transformants. Virulence was evaluated on detached leaves of rapeseed (Brassica napus) measured by the lesions diameter at 20°C for 72 h. Different letters in the graph indicate statistical differences, P = 0.01. (G) Sclerotial sizes of SSITL silenced transformants. Sclerotia were produced on the autoclaved carrot rods in 250 ml flasks at 20°C for 30 days.