Figure 6. Expression of PDF1.2 and PR-1 induced by transformants of S. sclerotiorum at locally inoculated leaves of A. thaliana at the early stage of infection.

The expression of PDF1.2 (A) and PR-1 (B) on leaves inoculated with silenced transformant A10 or with the wild-type strain Ep-1PNA367. The relative levels of transcript were calculated by the comparative Ct method. Expression on leaves of A. thaliana inoculated with pathogen for 0 hr was set as one. Transcript levels of GAPDH of Arabidopsis were used to normalize different samples. Bars represent means and standard deviations (three replications). (C) Lesions induced by transformant A10 and the wild-type strain Ep-1PNA367 on leaves observed at 20°C for 36 hr. Asterisks indicate statistical differences between the lesions diameter induced by A10 and Ep-1PNA367 (P<0.05).