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. 2013 Jan 14;8(1):e53901. doi: 10.1371/journal.pone.0053901

Figure 10. Enhanced susceptibility to SSITL silenced transformant A10 induced by the transient expression of SSITL in the host plants.

Figure 10

(A, B) The lesions induced by silenced transformant A10 and the wild-type strain Ep-1PNA367 on the leaves of tobacco (Nicotiana benthamiana) for 48 hr at 20°C. (C, D) The lesions induced by silenced transformant A10 and the wild-type strain Ep-1PNA367 on the leaves of A. thaliana Col-0 for 48 hr and 24 hr at 20°C, respectively. SSITL was expressed transiently in plants leaves by infiltrating with Agrobacterium GV3101 strain carrying SSITL expression vector. The leaves infiltrated with GV3101 carrying empty vector were selected as control (CK). Asterisks indicate statistical differences from the control (P<0.05). (E, F) Transcript levels of PDF1.2 and PR-1 in the SSITL transiently expressed A. thaliana leaves (black) after being respectively inoculated with silenced transformant A10 and the wild-type strain Ep-1PNA367 for 3, 6, 9 and 12 hr, with the plant leaves infiltrated with the GV3101 carrying empty vector being sampled for control (red). (G) Analysis the SSITL expression in the transiently expressed leaves of A. thaliana and tobacco (N. benthamiana) with RT-PCR. The leaves infiltrated with the GV3101 carrying empty vector were selected as control (CK). The A. thaliana GAPDH and N. benthamiana actin genes (see primers in Table 1) were used to normalize different samples.