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. 2013 Jan 14;8(1):e53947. doi: 10.1371/journal.pone.0053947

Table 1. Segregation of the S-RNase alleles in progenies of self-pollinations and outcrosses performed with the self-compatible cultivar ‘Katy’.

Seed parent(S-genotype)a Pollen parent(S-genotype) Populationname Nc S 1 S C S 2 S C S 1 S 2 S 2 S 2 S 1 S 1 S 1 S 4 S 2 S 4 Exp.Ratiod ? 2e P-value
Katy (S 1 S 2) Katy (S 1 S 2) ‘K×K’b 94 45 33 16 2∶1:1 6.32 (0.04)
Katy (S 1 S 2) Goldrich (S 1 S 2) 0
Goldrich (S 1 S 2) Katy (S 1 S 2) ‘G×K’ 26 12 10 4 2∶1:1 2.92 (0.23)
Harcot (S 1 S 4) Katy (S 1 S 2) ‘H×K’ 44 20 4 7 13 2∶1:1∶2 3.68 (0.30)
Katy (S 1 S 2) Canino (S 2 S C) ‘K×C’ 50 15 19 6 10 2∶2:1∶1 1.49 (0.69)
Canino (S 2 S C) Katy (S 1 S 2) ‘C×K’ 88 32 15 29 12 2∶1:2∶1 0.74 (0.86)

Observed S-RNase genotypes, expected segregation ratios and χ2 values obtained for each population are indicated.

a

S-genotypes for ‘Goldrich’, ‘Harcot’ and ‘Canino’ were prevously reported by Vilanova et al. (2005) and the S-genotype for ‘Katy’ was determined in this work.

b

‘K×K’ data correspond to three combined F2 populations obtained by self-pollinating ‘Katy’ in 2005, 2006 and 2010.

c

Obtained seedlings.

d

Expected ratios for a single mutation unlinked to the S-locus.

e

Observed ratios do not differ significantly from expected at P<0.05 (barring ‘Katy’ self-pollination at P>0.01).