Table 1. Segregation of the S-RNase alleles in progenies of self-pollinations and outcrosses performed with the self-compatible cultivar ‘Katy’.
Seed parent(S-genotype)a | Pollen parent(S-genotype) | Populationname | Nc | S 1 S C | S 2 S C | S 1 S 2 | S 2 S 2 | S 1 S 1 | S 1 S 4 | S 2 S 4 | Exp.Ratiod | ? 2e P-value |
Katy (S 1 S 2) | Katy (S 1 S 2) | ‘K×K’b | 94 | – | – | 45 | 33 | 16 | – | – | 2∶1:1 | 6.32 (0.04) |
Katy (S 1 S 2) | Goldrich (S 1 S 2) | – | 0 | – | – | – | – | – | – | – | – | – |
Goldrich (S 1 S 2) | Katy (S 1 S 2) | ‘G×K’ | 26 | – | – | 12 | 10 | 4 | – | – | 2∶1:1 | 2.92 (0.23) |
Harcot (S 1 S 4) | Katy (S 1 S 2) | ‘H×K’ | 44 | – | – | 20 | – | 4 | 7 | 13 | 2∶1:1∶2 | 3.68 (0.30) |
Katy (S 1 S 2) | Canino (S 2 S C) | ‘K×C’ | 50 | 15 | 19 | 6 | 10 | – | – | – | 2∶2:1∶1 | 1.49 (0.69) |
Canino (S 2 S C) | Katy (S 1 S 2) | ‘C×K’ | 88 | 32 | 15 | 29 | 12 | – | – | – | 2∶1:2∶1 | 0.74 (0.86) |
Observed S-RNase genotypes, expected segregation ratios and χ2 values obtained for each population are indicated.
S-genotypes for ‘Goldrich’, ‘Harcot’ and ‘Canino’ were prevously reported by Vilanova et al. (2005) and the S-genotype for ‘Katy’ was determined in this work.
‘K×K’ data correspond to three combined F2 populations obtained by self-pollinating ‘Katy’ in 2005, 2006 and 2010.
Obtained seedlings.
Expected ratios for a single mutation unlinked to the S-locus.
Observed ratios do not differ significantly from expected at P<0.05 (barring ‘Katy’ self-pollination at P>0.01).