Figure 2. Alanine scanning mutagenesis of the peripherin targeting sequence identifies V332 as the only residue essential for targeting.

Panels A–I show confocal images of transgenic frog retinas expressing the YFP-xRhoCTΔ5-xPer317–336 (green) variations containing single alanine substitutions of individual residues. (J) Multiple sequence alignment of peripherin targeting sequences from different species; residues with over 90% identity are highlighted yellow and the 100% conserved valine is highlighted orange. Abbreviations are: OS – outer segment, IS – inner segment, N – nuclei, ST – synaptic termini. Nuclei (blue) are stained with Hoechst. Scale bar 5 µm.