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. 2013 Jan 14;8(1):e50598. doi: 10.1371/journal.pone.0050598

Table 1. Cleavage site sequence of parental and recombinant APMV-4 viruses and pathogenicity in MDT and ICPI assays.

Virus Cleavage site MDTa ICPIb
Sequence Furin motif
wtAPMV-4 DIQPR ↓F - >168 0.00
rAPMV-4 DIQPR ↓F - >168 0.00
rAPMV-4/Fc type 3-Q RPQGR↓F - >168 0.00
rAPMV-4/Fc type 5-Q KRQKR↓F + >168 0.00
rAMPV-4/Fc BC RRQKR↓F + >168 0.00
rAMPV-4/Fc LaSota GRQGR ↓L - >168 0.00
rAMPV-4/Fc SV VPQSR↓F - >168 0.00
rAMPV-4/Fc PIV 1 NPQTR↓F - >168 0.00
a

Mean embryo death time (MDT): the mean time (h) for the minimum lethal dose of virus to kill all of the inoculated embryos. Pathotype definition: virulent strains, <60 h; intermediate virulent strains, 60 to 90 h; avirulent strains, >90 h.

b

Intracerebral pathogenicity index (ICPI): pathogenicity of NDV in 1-day-old SPF chicks following intracerebral inoculation. Pathotype definition: virulent strains, 1.5–2.0; intermediate virulent strains, 0.7–1.5; and avirulent strains, 0.0–0.7.