Table 2. PCR and sequencing results (L1 region).
| Sample ID | WTP site | Nested PCR | PaVE prototype strains | Nt identity | Hemi-nested PCR | PaVE prototype strains | Nt identity |
| 1528 | Torino | − | + | HPV120 | 98.9% | ||
| 1530 | Torino | + | HPV11 | 96.6% | + | HPV11 | 99.7% |
| 1530a | Torino | + | HPV11 | 99.1 | |||
| 1535 | Bari | − | + | HPV120 | 100% | ||
| 1539 | Palermo | + | HPV6 | 100% | + | HPV6 | 99.2% |
| 1542 | Palermo | + | HPV11 | 98.3% | + | HPV11 | 99.7% |
| 1543 | Cagliari | + | HPV16 | 100% | − | ||
| 1545 | Cagliari | + | HPV6 | 98.3% | + | HPV6 | 100% |
| 1545a | Cagliari | + | HPV6 | 100% | |||
| 1546 | Cagliari | − | + | HPV120 | 99.7% | ||
| 1547 | Cagliari | + | HPV6 | 98.3% | + | HPV6 | 99.5% |
| 1549 | Genova | + | HPV11 | 98.3% | + | HPV11 | 99.7% |
| 1550 | Genova | + | HPV6 | 97.4% | + | HPV6 | 99.2% |
| 1552 | Roma | − | + | HPV107 | 82.1% | ||
| 1556 | Genova | − | + | HPV16 | 100% | ||
| 1565 | Venezia | + | HPV6 | 98.3% | − | ||
| 1568 | Roma | + | HPV25 | 99.1% | + | HPV120 | 88.3%, |
| 1570 | Roma | − | + | HPV120 | 88% |
PCR was performed by Method A (L1 consensus region), using MY11/MY09 for the first cycle and either GP5+/GP6+ primers (nested reaction), or GP5+/My09 (hemi-nested reaction) for the second run. The samples positive at least to one assay are included in the table. It shows in columns from the left: the sample ID numbers and WTP sites (white panel), the nested PCR results (grey panel), the hemi-nested PCR results (dark grey panel). The table also reports the HPV genotypes more closely related to the query sequences, and the percentages of nucleotide (nt) identity toward these prototypes obtained by BLAST analysis. The letter (a) indicates the sequences obtained after cloning.