Table 3. PCR and sequencing results (E1 region).
Sample ID | WTP site | PaVE prototype strains | Nt identity | Unassigned sequences |
1528 | Torino | HPV145 | 94.9% | |
1529 | Torino | HPV9, HPV104, HPV113 | 90% | Unassigned (1) |
1530 | Torino | HPV9, HPV104, HPV113 | 90% | Unassigned (1) |
1531 | Bari | HPV9, HPV104, HPV113 | 90% | Unassigned (1) |
1532a | Bari | HPV9, HPV104, HPV113 | 90% | Unassigned (1) |
1532 | Bari | HPV9, HPV104, HPV113 | 89.2% | Unassigned (2) |
1533 | Bari | HPV9, HPV104, HPV113 | 89.2% | Unassigned (3) |
1534 | Bari | HPV9, HPV104, HPV113 | 90% | Unassigned (1) |
1535 | Bari | HPV9, HPV104, HPV113 | 90% | Unassigned (1) |
1537 | Palermo | HPV104 | 99.1% | |
1537a | Palermo | HPV20 | 100% | |
1538 | Palermo | HPV9, HPV104, HPV113 | 90% | Unassigned (1) |
1539 | Palermo | HPV9, HPV104, HPV113 | 90% | Unassigned (1) |
1540 | Palermo | HPV9, HPV104, HPV113 | 90% | Unassigned (1) |
1542 | Palermo | HPV9, HPV104, HPV113 | 90% | Unassigned (1) |
1543 | Cagliari | HPV9, HPV104, HPV113 | 89.2% | Unassigned (4) |
1543a | Cagliari | HPV 9 | 98.3% | |
1544 | Cagliari | HPV9, HPV104, HPV113 | 90% | Unassigned (1) |
1544a | Cagliari | HPV9, HPV104, HPV113 | 89.2% | Unassigned (5) |
1545 | Cagliari | HPV9, HPV104, HPV113 | 90% | Unassigned (1) |
1546 | Cagliari | HPV9, HPV104, HPV113 | 90% | Unassigned (1) |
1547 | Cagliari | HPV9, HPV104, HPV113 | 90% | Unassigned (1) |
1548 | Cagliari | HPV9, HPV104, HPV113 | 90% | Unassigned (1) |
1549 | Genova | HPV9, HPV104, HPV113 | 90% | Unassigned (1) |
1549a | Genova | HPV111 | 97.4% | |
1550 | Genova | HPV9, HPV104, HPV113 | 90% | Unassigned (1) |
1550a | Genova | HPV110 | 99.1% | |
1551a | Genova | HPV9, HPV104, HPV113 | 89.2% | Unassigned (6) |
1551b | Genova | HPV9 | 98.3% | |
1551c | Genova | HPV9 | 94% | |
1553a | Roma | HPV9, HPV104, HPV113 | 90% | Unassigned (1) |
1553 | Roma | HPV9, HPV104, HPV113 | 89.2% | Unassigned (7) |
1554 | Roma | HPV9, HPV104, HPV113 | 90% | Unassigned (1) |
1555 | Genova | HPV9, HPV104, HPV113 | 90% | Unassigned (1) |
1556 | Genova | HPV9, HPV104, HPV113 | 90% | Unassigned (1) |
1557 | Genova | HPV76 | 95.7% | |
1561 | Genova | HPV80 | 98.3 | |
1565 | Venezia | HPV9, HPV104, HPV113 | 90% | Unassigned (1) |
1566 | Venezia | HPV9, HPV104, HPV113 | 90% | Unassigned (1) |
1568 | Roma | HPV9, HPV104, HPV113 | 90% | Unassigned (1) |
1571 | Roma | HPV9, HPV104, HPV113 | 90% | Unassigned (1) |
PCR was performed by method D, using PM-A/PM-B primers (E1 consensus region). Only the positive samples are included in the table. In columns are shown from the left: sample ID numbers, sites of the WTP, the HPV genotypes more closely related to the query sequences, and the percentage of nucleotide (nt) identity toward these prototypes obtained by BLAST analysis. The sequences obtained from different clones are indicated by alphabetical code (ID sample “a”, “b”). Seven different sequences, showing less than 90% identity with more than one prototype, are indicated as unassigned sequences with progressive numbers (1–7) in brackets.