Fig. 2.
Single channel properties of native NRs. A: NR activities recorded from dissociated neurons segregate into two open probability (PO) ranges, which correlated roughly with culture age: early neurons (ENs; 4–33 DIV, n = 23) and late neurons (LNs; 25–50 DIV, n = 11). B: Scatterplot of single channel kinetic parameters for native (EN and LN) and recombinant (N1/2B and N1/2A) receptors. C: representative single channel current traces recorded from one EN receptor (top; 11 DIV) and one LN receptor (bottom; 26 DIV). D: histograms of closed (left) and open (right) event durations for the records illustrated in C (EN: green and LN: blue) overlaid with those measured in records from one recombinant N1/2B receptor (top) or N1/2A receptor (bottom; black). Overall probability distributions (thick solid lines) and individual closed (E1–E5) and open [fast (Of), low (OL), medium (OM), and high (OH)] kinetic components (thin solid lines) are also shown.