Fig. 5.
Effects of postsynaptic density protein (PSD)95 and neuropilin tolloid-like protein (Neto)1 on macroscopic responses of recombinant NRs. A: whole cell currents were elicited with 5-s applications of 1 mM glutamate from HEK-293 cells transfected with N1 and 2B (top) or with N1 and 2A (bottom) and in combination with PSD95 (left) or Neto1 (right). B: summary of changes in macroscopic desensitization kinetics observed in HEK-293 cells cotransfected NR subunits alone (black) and in conjunction with PSD95 (red) or Neto1 (blue). Iss/Ipk, steady-state (equilibrium) current-to-peak current; τD, time constant of macroscopic desensitization. *P < 0.05 relative to NR subunits alone (by Student's t-test).