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Immunoblot analysis of FOXP3 and GAPDH is shown as loading control. B. Representative fluorescent microscopy images of cells stained with antibodies to FOXP3 and probed with Alexa Fluor® 568 goat anti-rat IgG. Each slide was counterstained with DAPI and the overlay for each is shown. Hematoxylin and eosin (H&E) staining is shown for morphology of each cell line.
