Skip to main content
. 2013 Jan 14;8(1):e53649. doi: 10.1371/journal.pone.0053649

Figure 1. Schematic representation of experimental design employed in this study.

Figure 1

The full length of E. coli 16S rRNA gene was used as the reference scale. Fourteen blue arrow lines indicated all pyrotags analyzed in this study. S (L)-V1V2 represented the pyrotag contained 16S V1V2 region derived from a Short (Long) amplicon. Four small PCR fragments covered 16S V1V2, V3V4, V5V6, and V7V8V9 regions were defined as Short amplicons. The remainder three large PCR fragments targeted 16S regions of V1–V4, V3–V6, and V5–V9 were defined as Long amplicons.