Figure 6. Response of hxk1 response to cell wall perturbing agent and high temperature.

Wild-type strain, hxk1 mutant and revertant strains were grown in YPD for 8 hr at 30°C and 10-fold dilution series was spotted on YPD, YPD+ Nikkomycin Z (20 µg ml⁻¹), YPD₊ Congo Red (100 µg ml⁻¹) and YPD+Calcofluor white (30 µg ml⁻¹) plates at indicated concentrations(A and B). For temperature shift experiments, spotted plates were kept at 30°C and 42°C.