Skip to main content
. 2013 Jan 14;8(1):e53638. doi: 10.1371/journal.pone.0053638

Figure 7. HXK1 localized in the cytoplasm, nucleus and mitochondria in condition specific manner.

Figure 7

Sub-cellular localization of Hxk1p-GFP in wild-type cells induced in GlcNAc 2.5 mM or 2% and non-fermentative carbon sources like ethanol or glycerol, 5%. iHXK1-GFP along with wild type (control) grown for 6 h in YPD at 30°C and then, washed in water and induced in GlcNAc 2.5 mM (salt base) or 2%. Cells were visualized either by bright field or by epifluorescence at 1 hr, 2.5 hr and 4 hr in 2.5 mM GlcNAc (A), and 4 hr in 2% GlcNAc (B) after induction. The nuclear localization of Hxk1p was further confirmed by western blotting analysis. iHXK1-Myc strain was induced in 2.5 mM GlcNAc (C) or 2% glucose (D), nuclear and total cellular fractions were checked for presence of hxk1p with help of anti c-myc antibody. The purity of nuclear fraction (free of cytoplasmic contamination) was checked by using Anti-GAPDH antibody specific for cytoplasmic fraction. To check localization in non-fermentable carbon sources, cells were grown for 6 h in YPD at 30°C, washed in water and induced in YNB w/o amino acid with 5% ethanol (E). Hxk1 localization is marked with white arrowheads.