Table 1.

In situ hybridization for β2 mRNA by brain region and genotype

Brain Region	WT cpm/mg wet wt	HT cpm/mg wet wt	MT cpm/mg wet wt	ANOVA Results
Cortex	1081 ± 30	1168 ± 23	1105 ± 102	F2,9= 0.51, p=0.62
Frontal cortex	932 ± 44	1067 ± 24	1002 ± 40	F2,9= 3.33, p=0.08
Olfactory bulbs	1262 ± 65	1569 ± 98	1255 ± 735	F2,9=0.17, p=0.84
Interpeduncular nucleus	2097 ± 149	2136 ± 86	2008 ± 449	F2,9= 0.05, p=0.94
Medial habenula	7654 ± 1012	6764 ± 712	6015 ± 735	F2,9= 0.97, p=0.41
Olfactory tubercles	805 ± 42	937 ± 44	1004 ± 64	F2,9= 3.94, p=0.06
Striatum	692 ± 44	756 ± 29	784 ± 60	F2,9=1.05, p=0.39
Dorsolateral geniculate	2406 ± 350	2737 ± 110	2374 ± 221	F2,9= 0.66, p=0.54
Medial geniculate	1871 ± 179	2065 ± 66	1671 ± 164	F2,9=1.84, p=0.21
Superior colliculus	1535 ± 174	1707 ± 39	1710 ± 234	F2,9= 0.34, p=0.72
Inferior colliculus, dorsal cortex	1717 ± 81	1625 ± 58	1547 ± 176	F2,9= 0.53, p=0.61
Thalamus	2079 ± 149	2376 ± 104	1882 ± 197	F2,9=2.58, p=0.13
Thalamus, anteroventral nucleus	2571 ± 156	2733 ± 118	2445 ± 303	F2,9= 0.48, p=0.63
Ventral tegmental area	2209 ± 116	2208 ± 45	2465 ± 368	F2,9= 0.44, p=0.66
Substantia nigra	1781 ± 46	1707 ± 138	1397 ± 108	F2,9=3.79, p=0.06

Data show in situ hybridization for [³⁵S] β2 cRNA in mouse brain regions of all three β2VL genotypes. Hybridization was performed as described in the methods section. Specific hybridization was quantitated utilizing standards of known activity and is represented in terms of cpm/mg wet wt. Values are the mean ± SEM measured in four different mice.