Table 2.

β2 protein expression by region and genotype using mAb 270

Brain Region	WT fmol/mg wet wt	HT fmol/mg wet wt	MT fmol/mg wet wt	ANOVA Results
Cortex	1.02 ± 0.29	0.78 ± 0.18	0.56 ± 0.31	F2,9=0.75. p=0.50
Frontal cortex	1.38 ± 0.20	1.05 ± 0.20	0.86 ± 0.26	F2,9=1.47, p=0.28
Olfactory bulbs	0.66 ± 0.16	0.43 ± 0.16	0.49 ± 0.08	F2,9=0.74, p=0.50
Interpeduncular nucleus	4.56 ± 0.69	4.80 ± 0.33	4.07 ± 0.78	F2,9=0.35, p=0.72
Olfactory tubercles	0.76 ± 0.20	0.78 ± 0.20	0.46 ± 0.12	F2,9=1.02, p=0.40
Striatum	1.65 ± 0.23	1.50 ± 0.20	1.27 ± 0.21	F2,9=0.80, p=0.48
Medial geniculate	2.82 ± 0.18	3.40 ± 0.22	2.32 ± 0.30	F2,9=5.09, p=0.03*
Dorsolateral geniculate	4.77 ± 0.59	4.63 ± 0.33	3.50 ± 0.61	F2,9=1.76, p=0.23
Ventrolateral geniculate	3.72 ± 0.22	4.15 ± 0.33	3.24 ± 0.33	F2,9=2.36, p=0.15
Superior colliculus, optic nerve layer	3.00 ± 0.46	3.84 ± 0.39	3.42 ± 0.68	F2,9=0.10, p=0.90
Superior colliculus, superficial gray	3.72 ± 0.54	4.90 ± 0.54	4.32 ± 0.78	F2,9=0.88, p=0.45
Inferior colliculus, dorsal cortex	1.11 ± 0.15	1.56 ± 0.32	1.37 ± 0.46	F2,9=0.43, p=0.67
Thalamus	2.49 ± 0.36	2.93 ± 0.21	2.29 ± 0.33	F2,9=1.20, p=0.35
Thalamus, anteroventral nucleus	2.82 ± 0.26	2.95 ± 0.12	2.06 ± 0.37	F2,9=3.17, p=0.09
Trigeminal nucleus	1.91 ± 0.45	1.75 ± 0.12	1.45 ± 0.23	F2,9=0.60, p=0.57
Medial ventricular nucleus	1.03 ± 0.25	1.07 ± 0.20	0.87 ± 0.15	F2,9=0.27, p=0.77

β2 nAChR subunit protein levels were measured with binding of [¹²⁵I]-mAb 270 as described in Methods. Binding was quantitated using [¹²⁵I]-standards of known activity and expressed as fmol/mg wet wt based on specific activity of the [¹²⁵I]-mAb 270. Results are the mean ± SEM from four individual mice per genotype. Regions with significant differences by one-way ANOVA are shown in bold type.