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. 2001 Jul 3;98(14):7958–7963. doi: 10.1073/pnas.131207398

Figure 4.

Figure 4

MLN leukocyte analysis after infection with M. tuberculosis. Leukocytes from MLNs were isolated from CCR2−/− and CCR2+/+ mice and analyzed by flow cytometry on days 9, 11, 13, and 17 after infection. The cells were stained with fluorescent antibodies to F4/80 (macrophages), CD11c (bright; DCs), CD4, and CD8. Shown are the mean cell numbers for each cell type for four to six CCR2−/− and CCR2+/+ mice on each day. Error bars represent the standard deviation from the mean (*, P ≤ 0.05; **, P ≤ 0.01). Analysis of another macrophage marker (CD11b+Gr-1−) yielded results similar to those obtained with F4/80 (data not shown).