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IFN-γ produced by MLN cells after infection with M. tuberculosis. MLN cells from CCR2−/− and CCR2+/+ mice were isolated and re-stimulated in vitro with PPD (10 μg/ml) on days 9, 13, and 17 after infection. After 48 h, the supernatants were assayed for IFN-γ by ELISA. The individual values for CCR2−/− and CCR2+/+ mice from one of three similar experiments (*, P ≤ 0.05; **, P ≤ 0.01) are shown.
