Table 1.

Inhibition of mPTP opening (cyclosporin A) or caspase activation (qVD-OPH) does not prevent ΔΨ_m depolarization following heat stress

	TMRM Fluorescence, % no-stress control
Treatment	Cyclosporin A	No drug
No stress	83.8 ± 6.3	100 ± 7.5
Time post-stress, h
0.2	55.3 ± 2.6	56.8 ± 1.44
6.0	59.4 ± 3.2	70.6 ± 4.0
12.0	30.8 ± 2.9	32.6 ± 2.3
24.0	31.1 ± 2.3	39.2 ± 2.6

	TMRM Fluorescence, % no-stress control
Treatment	qVD-OPH	No drug
No stress	88.0 ± 6.4	100 ± 3.9
Time post-stress, h
1.5	83.0 ± 2.3	84.2 ± 2.7
4.5	41.3 ± 2.3	34.8 ± 2.47
7.0	49.9 ± 4.8	41.6 ± 3.92
24.0	21.5 ± 4.6	29.7 ± 8.7

Platereader measurements of TMRM fluorescence in sister cultures in the presence or absence of the indicated drug. N = 12–18 culture wells. 1 μM cyclosporin A or 20 μM qVD-OPH was added 30 min before the stress; cyclosporin A was also supplemented 2 h before each post-stress measurement. Cyclosporin A findings were confirmed in 3 additional experiments; qVD-OPH findings were confirmed in 2 additional experiments.