Table 1.
TMRM Fluorescence, % no-stress control |
||
---|---|---|
Treatment | Cyclosporin A | No drug |
No stress | 83.8 ± 6.3 | 100 ± 7.5 |
Time post-stress, h | ||
0.2 | 55.3 ± 2.6 | 56.8 ± 1.44 |
6.0 | 59.4 ± 3.2 | 70.6 ± 4.0 |
12.0 | 30.8 ± 2.9 | 32.6 ± 2.3 |
24.0 | 31.1 ± 2.3 | 39.2 ± 2.6 |
TMRM Fluorescence, % no-stress control |
||
---|---|---|
Treatment | qVD-OPH | No drug |
No stress | 88.0 ± 6.4 | 100 ± 3.9 |
Time post-stress, h | ||
1.5 | 83.0 ± 2.3 | 84.2 ± 2.7 |
4.5 | 41.3 ± 2.3 | 34.8 ± 2.47 |
7.0 | 49.9 ± 4.8 | 41.6 ± 3.92 |
24.0 | 21.5 ± 4.6 | 29.7 ± 8.7 |
Platereader measurements of TMRM fluorescence in sister cultures in the presence or absence of the indicated drug. N = 12–18 culture wells. 1 μM cyclosporin A or 20 μM qVD-OPH was added 30 min before the stress; cyclosporin A was also supplemented 2 h before each post-stress measurement. Cyclosporin A findings were confirmed in 3 additional experiments; qVD-OPH findings were confirmed in 2 additional experiments.