Fig. 6.
Activation of 5-HT2CR inhibits potassium currents. To better characterize the ionic basis of CP809101 on membrane properties, a depolarizing ramp protocol (−110 to −40 mV, 200 ms; A and C, top) was performed and membrane currents were recorded. A: compared with control (black trace), CP809101 (dark gray trace) decreased slope conductance which reversed at ∼90 mV. B: the subtracted CP809101-sensitive curve reverses near the K+ equilibrium potential. C: barium (light gray trace) alone decreased membrane conductance from control (black traces). Barium also prevented the change in slope conductance by CP809101 (dark gray trace). D: the subtracted barium-sensitive current reversed ∼90 mV, while the CP-sensitive current was eliminated in barium. E and F: barium prevented the CP809101-induced alterations in input resistance (E; from −60 mV to −65 mV) and TS-EPSCs (F; 0.5 Hz).