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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Mol Microbiol. 2012 Dec 10;87(2):382–393. doi: 10.1111/mmi.12105

Fig. 3.

Fig. 3

Elimination of RNase III suppresses the Nus cold-sensitive ribosome subunit phenotype. Shown are sedimentation profiles of ribosomal subunits prepared from W3110 derivatives NB478 (rnc < >cat) (panel A), NB840 (nusB < >cat, rnc< >spc) (panel B), and NB876 (nusAcs10, rnc< >cat) (panel C) labeled with [3H]-uridine at 20°C. Cells were labeled and extracts prepared and run on gradients as shown for Fig. 2 and described in Methods.