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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Mol Microbiol. 2012 Dec 7;87(2):318–337. doi: 10.1111/mmi.12100

Figure 7.

Figure 7

SCAM predicted membrane topology of AgrB and revised AIP biosynthesis model. Panel A, the topology model is based on interpretation of the SCAM assay results shown in Figures 5 and 6. Sites of mutations that decreased AIP production are shaded gray. Residues K129–K131, which disrupted a later step in AgrD processing, are boxed. Panel B, a model for AIP biosynthesis was previously reported (Thoendel & Horswill, 2009), and this model has been revised using the new AgrB topology assignments.