Table 1.
Strains and plasmids used in this study.
| Strain or Plasmid | Genotype/Description | Resistance | Source or Ref. |
|---|---|---|---|
| Escherichia coli | |||
| ER2566 | Protein expression strain | None | New England Biolabs |
|
| |||
| Staphylococcus aureus | |||
| RN4220 | Restriction modification deficient | None | (Kreiswirth et al., 1983) |
| ROJ143 | Δagr∷Erm pAgr-lux / pAgrC1agrA | Cam | (Jensen et al., 2008) |
| AH845 | USA300 LAC | Tet | (Voyich et al., 2005) |
| AH1263 | USA300 LAC (Erythromycin sensitive) | Tet | (Boles et al., 2010) |
| AH1292 | AH1263 Δagr∷tetM | Tet | (Benson et al., 2011) |
| AH2157 | USA300 LAC spa∷kan | Kan, Erm | Tim Foster |
| AH1677 | AH845 / pDB59 (agr type I reporter) | Cam | (Kirchdoerfer et al., 2011) |
| AH430 | SA502a / pDB59(agr type II reporter) | Cam | (Malone et al., 2007) |
| AH1747 | MW2 / pDB59 (agr type III reporter) | Cam | (Kirchdoerfer et al., 2011) |
| AH1872 | MN EV / pDB59 (agr type IV reporter) | Cam | (Kirchdoerfer et al., 2011) |
|
| |||
| Plasmids | |||
| pEPSA5 | Xylose inducible shuttle vector | Amp, Cam | (Forsyth et al., 2002) |
| pAgrD1 | pEPSA5-agrBD | Amp, Cam | (Thoendel & Horswill, 2009) |
| pDB59 | agr P3-YFP | Amp, Cam | (Yarwood et al., 2004) |
| pCOLADuet-1 | Expression vector | Kan | Novagen |
| pCOLADuet-T7agrB-His6agrD | Wild-type tagged AgrB and AgrD | Kan | (Thoendel & Horswill, 2009) |
| pCOLADuet-T7agrB(C84S)-His6agrD | Non-proteolytic AgrB and AgrD | Kan | (Thoendel & Horswill, 2009) |
| pCOLADuet-agrB(C84S)-His6agrD(δ 14aa) | His6AgrD with C-terminal tail deleted | Kan | (Thoendel & Horswill, 2009) |
| pCOLADuet-T7agrB(C84S)-HiS6agrD(δ26aa) | His6AgrD with AIP and C-terminal tail deleted | Kan | (Thoendel & Horswill, 2009) |
| pCOLADuet-T7agrB-His6agrD(C28A) | Wild-type tagged AgrB and His6AgrD with AIP cysteine mutated | Kan | (Thoendel & Horswill, 2009) |
| pEPSA5-T7agrB | Xylose inducible T7-tagged AgrB | Amp, Cam | This study |
| pEPSA5-His6agrB | Xylose inducible His6-tagged AgrB | Amp, Cam | This study |
| pEPSA5-T7agrB-His6agrB | T7-tagged AgrB and His6-tagged AgrB | Amp, Cam | This study |
| pEPSA5-His6saeS | Xylose inducible His6-tagged SaeS | Amp, Cam | This study |
| pEPSA5-T7agrB-His6saeS | T7-tagged AgrB and His6-tagged SaeS | Amp, Cam | This study |